Synthesis of Nucleic Acid Therapeutics – End-to-end Workflow & Process Optimisation
Time: 5:00 pm
day: Day One PM
Details:
- Phi29 DNA polymerase utilizes rolling circle amplification to produce cell-free linear DNA template for RNA synthesis with the flexibility to work at the RUO and GMP grade
- Therapure IVT reagents were able to consistently synthesise RNA at multiple volumes with Therapure GMP reagents reaching 1 gram of RNA at the 100 mL synthesis scale
- Design of experiments were used to determine the buffer components and reagent proportions that result in optimal IVT performance