Advancing RNA Therapeutics Development: Novel Reagents for DNA Template Production by Rolling Circle Amplification
Time: 4:30 pm
day: Day 1 PM
Details:
- The rolling circle amplification (RCA) is an isothermal amplification technique that shows promise for generating DNA template for in vitro transcription (IVT) reaction used in therapeutic RNA production. Compared to using bacterial plasmids as DNA templates, RCA offers several advantages as speed, simplicity and safety
- For mRNA therapeutics development the AOF (animal origin free) reagents are of interest due to the reduced risk for impurities associated with animal origin materials. Recently, we have developed the TheraPure 29 DNA polymerase (AOF), and investigated the enzyme’s performance under various RCA conditions, including different reaction times, temperatures, different sizes of DNA templates. RCA generated DNA template was used for IVT reaction and compared to plasmid DNA. RCA scalability was evaluated
- Our results support the use of TheraPure 29 DNA polymerase and other Thermo Fisher Scientific reagents for DNA template preparation by RCA for RNA based therapeutics development
